Abstract
The study investigated the use of clove oil as anaesthetic in Clarias gariepinus. One hundred and eight juvenile fish (40.6±1.52 to 50.0±3.20 g body weight and 10.0±1.35 to 18.67±2.81 cm total length) were treated with (0, 65, 75, 85, 95, and 105 mg/L) of clove bud extract per litre of water in plastic tanks in triplicates. Blood samples were taken for haematology, plasma biochemistry, and serology parameters. The results show that stage 3 induction and recovery were achieved in fish treated with 95mg/l and 105mg/l of E. caryophyllata per litre of water. There was a strong relationship between induction/recovery periods and concentration relationship; y=57. 91-27.45 induction time (x), R² = 0.907/concentration of oil (y) =50. 08-29.41 recovery time (x), R² = 0.921). Fish treated higher concentrations (85 to 105 mg/l) had higher haematology and blood enzyme parameters, but similar to lower plasma biochemistry than the control. Fish treated with 95 and 105 had PVC (27.00±1.00 and 32.33±1.15%), Hb (8.73±0.31 and 11.07±0.75dl), RBC (3.25±0.50 and 3.45±0.04 ), WBC (18.72±0.44 and 18.77±0.18), AST (194.17±10.11 and 197.33±7.37 µl), ALP (232.17±63.64 and 270.00±66.30 µl), urea (8.30±0.72 and 7.13±0.23µl), creatinine (0.72±0.19 and 0.83±0.15mg/dl), glucose (277.50±90.36 and 300.67±30.62mg/dl) and cholesterol (243.83±92.57 and 279.33±41.02mg/dl) higher than the control PVC (24.78±4.31%), Hb (7.96±1.36 cl), RBC (1.72±0.87 ), WBC (17.10±1.15 ), AST (181.06±41.75µl), ALP (187.83±51.22µl), urea (6.86±0.53µl), creatinine (0.68±0.15mg/dl), glucose (232.44±104.23mg/dl) and cholesterol (184.33±92.57mg/dl) respectively. There were statistical differences (p<0.05) in the haematology, plasma biochemistry and blood enzymes of fish. E. caryophyllata oil is a suitable, cheaper, and a naturally-derived anaesthetic material for C. gariepinus juveniles.
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