Oxidative Stress and Antioxidant Response in the Giant African Catfish (Heterobranchus bidorsalis Geoffroy Saint-Hilaire, 1809) under Chronic Paraquat Exposure
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Keywords

Paraquat
Oxidative stress
Antioxidant response
Heterobranchus bidorsalis

Abstract

The study assessed oxidative stress and antioxidant response parameters in the liver, kidney and gills of the Giant African catfish; Heterobranchus bidorsalis. Fish were exposed to different Paraquat concentrations of 0.0mg L-1, 0.69mg L-1, 1.37mg L-1 and 2.75mg L-1 for 28 days. Oxidative stress (Lipid peroxidation) was measured as malondialdehyde (MDA).  Antioxidant response was measured as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GH-Px). The herbicide stress caused significant (P<0.05) increase in the MDA, SOD and CAT activity in all the organs examined except in the kidney in which the CAT was significantly decreased. The GH-Px activity, decreased in these organs and the changes in all these parameters were concentration dependent.  These parameters significantly differ from the control (0.0mg L-1) with   no paraquat concentration. The study suggests that Paraquat induces oxidative stress in exposed fishes.  This antioxidant response has the potential to serve as a biomarker for the monitoring of bipyridyl herbicides in aquatic environments.

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