Effect of Filleting and Peeled Undeveined Pre-Processing Methods on the Microbial Load of Frozen Nigerian Tongue Sole Fish (Cynoglossus Browni) and Brown Shrimps (Parapenaeopsis Atlantica) in a Fish Processing Plant
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Keywords

Microbial load
Pre-processing
Brown Shrimps
Nigerian Tongue Sole Fish
Preparation methods

Abstract

The study examined the impact of filleting and peeled undeveined pre-processing methods on the microbial load of Cynoglossus browni and Parapenaeopsis atlantica in a fish processing plant. These pre-processing steps converted P. atlantica and C. browni into ready-to-cook products, such as peeled undeveined shrimps and sole fish fillets. The study involved taking twenty samples of both P. atlantica and C. browni, before and after pre-processing, to the laboratory for standard microbiological analysis. The results showed that the total plate count and Staphylococcus aureus count in P. atlantica and C. browni significantly reduced after applying the peeled undeveined and filleting pre-processing methods, except for yeast and mold, which had an increase in its count. The study also found that the microbial count of Staphylococcus aureus in frozen Sole fish decreased from 108CFU/g to 44CFU/g in sole fillets. Notably, the pre-processing methods were most effective on P. atlantica, reducing the total plate count of frozen brown shrimps from 87123CFU/g to a total plate count of 1159CFU/g in peeled undeveined shrimps. However, the pre-processing method was also least effective on P. atlantica, with a 14.5% increase in yeast and mold of frozen brown shrimps from 821CFU/g to yeast and mold of 1186.5CFU/g in PUD shrimps. Additionally, the study found that for both species in the frozen and pre-processed states, the most probable number had less than 3.0 CFU/g. The study also revealed that Escherichia coli, Salmonella spp, Shigella spp, and Vibrio parahaemolyticus were not detected. Conclusively, the microbial counts were within the limit recommended by the European Commission, indicating that these products are safe for consumption.

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References

Abowei, J. & Tawari, C.C. (2011). Some basic principles of Fish Processing in Nigeria. Asian Journal of Agricultural Sciences 6(6), 437-b452.

Adeboyejo, O. A, Clark, O. E. & Fajana, O. P. (2021). “Microbial Load In Five Demersal Marine Fin and Shell Fishes from Nigerian Coastal Waters”. Ad Oceanogr & Marine Biol. 2 (4). AOMB.MS.ID.000543. DOI: 10.33552/AOMB.2021.02.000543. 2(4), 1-7.

Akinwunmi, F. O. & Adegbehingbe K. T. (2015). Microbiological Analysis of Three Smoke Fish obtained from Ondo State, Nigeria. Food and Public Health. 5(4),122-126. Doi: 10.5923/j.fph.20150504.04

Assogba, H., Salifou, C.F.A., Gilbert, H., Raoul, A., Mahamadou, D., Antoine, C., Souaibou, F. Issaka, Y. & Abdou, K. (2018). Effect of Processing and Preservation Processes on the microbiological quality of Coptodon guineensis and Sarotherodon melanotheron in South Benin. International Journal of Microbiology and Mycology 7(4), 2309-4796.

Bacteriological Analytical Manual (BAM) (2001). Bacteriological Analytical Manual. Laboratory Methods US Food & Drug Administration.\ FDA) 8th Edition Chapters 3-18.

Bykowski, P. & Dutkiewicz, D. (1996) Freshwater fish processing and equipment in small plants. FAO Fisheries Circular No. 905. Rome, FAO. 1996., p.59.

De Sousa, G.B., Tamagnini, L. M., Olmos, P. D. & Gonzalez, R. D. (2002). Microbial enumeration in ready-to-eat foods and their relationship in good manufacturing practice. J. Food Safety, 22:27-38.

Eranda, D.H.U., Chaijan, M, Uysal-Unalan, I, Panpipat, W, Naik, A.S., Dib, A. L, Karnjanapratum, S. & Gagaoua, M. (2024). Biopreservation of pre-processed fresh fish by bio-based coatings: A single strategy with multiple benefits towards waste prevention. Food Bioscience. 58.2024. 103696. ISSN 2212-4292. https://doi.org/10.1016/j.fbio.2024.103696.

European Commission (EC) (2005). Discussion Paper on strategy for setting microbiological criteria for foodstuffs in Community legislation. Health and Consumer Protection Directorate-General. Directorate D- Food Safety: production and distribution chain D2- Biological risks. Brussels 8.3.2005 SANCO/1252/2001 Rev. 11:22-26.

Fajana, O.P. & Mekuleyi, G. (2019). Implementing the HACCP concept in Nigeria Tongue Sole fish (Cynoglossus browni) fillets and mince production in a Fish Processing Plant for Export (2019). International Journal of Food Sciences. 2(1),1-16. DOI: 10.47604/ijf.973.

FAO (2011). Risk Assessment of Vibrio parahaemolyticus in Seafood: Interpretative Summary and Technical Report. Microbiological Risk Assessment Series (16),193, Rome.

Jag, P., Shukka, B.M.N., Ashish, K. M., Hari O.V., Gayati, P. & Amitha (2018). A review of the role of fish in human nutrition with special emphasis on essential fatty acids. International Journal of Fisheries and Aquatic Studies. 6(2): 427-430. www.fisheriesjournal.com.

Mohamed M. S., El-barbary, Y. A. & Ahmed, O. M. (2022). Assessment of Performance, Microbial Community, Bacterial Food Quality, and Gene Expression of White Leg Shrimp (Litopenaeus vannamei) Reared under Different Density Biofloc Systems. Aquaculture Nutrition, 2022: 13 Article ID 3499061., https://doi.org/10.1155/2022/3499061.

More, A. & Likhar, V. (2020). Fish Processing: Product and by-product, processing, and marketing. International Journal of Fisheries and Aquatic Studies; 8(1),74-77. E-ISSN: 2347-51229. P-ISSN: 2394-0506. www.fisheriesjournal.com.

Nwaigwe, U. (2017). Fish preservation and processing. Journal of Food CyTA. www.researchgate.net/publication/316918904_fish_preservationand_processing.

Okonko, I.O., Ogunnusi, T.A., Ogunjobi, A. A., Adedeji, A.O., Adejoye, O.D., Babalola, E.T., & Ogun, A. A. (2008). Microbial studies on frozen shrimps processed in Ibadan and Lagos, Nigeria. Academic Journals Scientific Research and Essay, 3(11), 537-546. http://www.academic journals.org/SRE. ISSN 1992-2248.

Okonko, I.O., Adejoye, O.O., Ogun, A.A., Ogunjobi, A.A., Nkang, A.O. & Adebayo-Tayo, B.C. (2020). Hazards analysis critical control points (HACCP) and microbiology qualities of seafoods as affected by handler’s hygiene in Ibadan and Lagos, Nigeria. African Journal of Agriculture and Food Security. ISSN 2375-1177. 8 (2), 001-016. www.internationalscholarsjournal.

Olokun Pisces Limited HACCP Plan (2022/2023). Hazard Analysis Critical Control Point Plan for Land-based Processing 2022/2023 of Olokun Pisces Limited Processing Plant. 61 – 64. Updated on September 15, 2022.

Rahman, M. M., Rahman, F., Afroze, F.F., Yesmin, F., Fatema, K., Das Kanta, K., & Noor, R. (2012). Prevalence of Pathogenic Bacteria in Shrimp samples collected from Hatchery, Local Markets, and the Shrimp Processing Plant. Bangladesh J. Microbiol, 29(1), 7-10. June 2012.

Sampels, S (2015). The effects of processing technologies and preparation on the final quality of fish products. Trends in Food Science & Technology. 44(2),131-146, ttps://doi.org/10.1016/j.tifs.2015.04.003

Sheng, L. & Wang, L. (2020). The microbial safety of fish and fish products: Recent advances in understanding its significance, contamination sources, and control strategies. Comprehensive Reviews in Food Science and Food Safety Volume 20, Issue 1/p. 738-786.

Sohana, A.S. & Ekramul, K. Md. (2016). Microbiological Quality Assessment of Frozen Fish and Fish Processing Materials from Bangladesh. International Journal of Food Science. 2016: 6 Article ID 360568.

Subramanian, A.T. (2007). Effect of processing on bacterial population of cuttlefish and crab and determination of bacterial spoilage and rancidity developing o frozen storage. Journal of Food Processing and Preservation, 31: 13-31, 10.1111/j. 1745-4549.2007. 00101.X

Udo, I., Udoh, D. & Umoren, N. (2022). Impacts of Processing Methods on the Microbial Loads and Proximate Composition of Tagelus adansonii Bosc, 1801 (Mollusca: Bivalvia, Solecurtidae) in Mangrove swamps of Iko Estuary, Southeast, Nigeria. Global Scientific Journals. 10(2), 320-9186. www.globalscientificjournal.com.

Wikipedia (2022). Fish Processing. https://en.wikipedia.org/wiki/Fish_processing updated and edited on 16 September 2022. www.alimentarium.org (2024). Preparing fish. Preparing a fresh fish requires knowledge and dexterity. Alimentarium, a Nestle Foundation. https://www.alimentarium.org/en/fact-sheet/preparing fish.
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