Abstract
A ten-week feeding experiment was carried out to examine the effect of replacing dicalcium phosphate (DCP) with milled Quail Egg Shells (QES) on the growth performance, haematology, and biochemical parameters of Clarias gariepinus juveniles. A control experiment was set up with a 39% crude protein diet. One hundred and fifty juveniles of Clarias gariepinus with a weight range of 213.0 – 215.0 g (mean weight: 213.6 ± 0.55) were fed for ten weeks on, 0, 25, 50, 75, and 100% inclusion levels of QES as substitute for dicalcium phosphate. The results show that the highest mean weight gain (MWG) was recorded in fish fed 50% QES-based diet (44.06±7.09 g), while the lowest weight gain was recorded on the fish-fed control diet (25.62±10.16 g). In the haematological parameters, the fish fed 50% eggshell had the highest packed cell volume (PCV) (38.90±0.87 g/l) and the white blood cell count (WBC) (162.60±2.71 x 109 G), while the biochemical analysis showed that the fish fed 25% eggshell diet had the best values (13.88 ± 0.62 mg/gl; 2.00 ± 2.00 mg/gel) in urea and alkaline phosphatase (ALP) respectively. It is concluded, based on the growth performance and haematological parameters that the 50% dicalcium phosphate can be replaced by milled quail egg shells in the diet of Clarias gariepinus juveniles... Although it showed a slight decrease in the biochemical parameters, it had no negative impact on the health status of the species.
References
Adeyemo, O.K., Agbede, S.A., Olaniyan, A.O. & Shoag, O.A., (2003). The haematological response of Clarias gariepinus to changes in acclimation temperature, African Journal of Biomedical Research, 6:105–108.
Adikwu, I. (1992). Dietary carbohydrate utilization in Tilapia (Oreochromis niloticus), Journal of Agriculture Science and Technology 2(1): 33-37.
Ayoola, S.O. (2011). Haematological characteristics of Clarias gariepinus (Burchell, 1822) juveniles fed with poultry hatchery waste. Iranica Journal of Energy and Environment 2: 18-23.
Basten, G. (2010). Introduction to clinical biochemistry: Interpreting blood results. Graham Basten and Ventus Publishing ApS, Leicester. 57Pp.
Boyd, C.E. (1979). Water Quality in Warm Water Fish Ponds. Agric. Exp. Sta. Auburn University Craftmaster Printers Inc., Opelika, Alabama, 359Pp.
Das, B. C. (1965). Age related trends in the blood chemistry and haematology of the Indian carp (Catla catla). Gerontologia 10:47– 64.
Efthimiou, S., Divanach, P. and Rosenthal, H. (1994). Growth, food conversion and agonistic behaviour in common dentex (Dentex dentex) juveniles fed on pelleted moist and dry diets. Aquatic Living Resources, 7:267 – 275.
Engel, D. M. and Davis, E. M. (1964). Relationship between activity and blood composition in certain marine teleosts. Copeia 3:586–587.
Eyo, A. A. (2002). Chemical composition of amino acid content of the commonly available feedstuffs sued in Nigeria. In Eyo, A. A. (Ed) Fish Nutrition and Feed Technology. Published by the fisheries Society of Nigeria (FISON) 15-26.
Fagbenro, O. A. and Adebayo, T. (2002). A review of the animals and aquafeed industries in Nigeria. In: Livestock in livestock and fish feeds in sub-Saharan African, compiled by tom Hechi. FAO Fisheries Technical paper, No. 7. FAO, Rome. 21Pp.
Federal Department of Fisheries (2005). Fisheries Statistics of Nigeria. 2-43
Haruna, B. (1997). Studies on utilization of Agro and Agro-allied wastes in the Nutrition of the African Catfish, (Clarias gariepinus) Burchell- 1822. PhD Thesis, Department of Biological Sciences, Bayero University, Kano, 38-93.
Omitoyin, B. O. (2007). Plasma Biochemical Changes in Clarias gariepinus (Burchell, 1822) Fed Poultry Litter. Asian Journal of Animal Sciences, 1: 48-52.
Omoniyi, I., Agbon, A. O. and Sodunke, S. A. (2002). Effect of lethal and sub-lethal concentrations of tobacco (Nicotiana tobaccum) leaf dust extract on weight and haematological changes in Clarias gariepinus (Burchell). Journal of Applied Sciences and Environmental Management, 6(2): 37-41.
Onyewuchi, U. U., Offor, I. R. and Okoli, C. F. (2013). Profitability of Quail bird and egg production in Imo State. Nigerian Journal of Agriculture, Food and Environment, 9(1):40-44.
Osuigwe, D. I., Nwosu C. and Ogunji J. O. (2007). Preliminary Observations on some Haematological Parameters of Juvenile Heterobranchus longifilis fed different dietary Levels of Raw and Boiled Jackbean (Canavalia ensiformis) Seed Meal. Tropentag University of Kassel-Witzenhausen and University of Göttingen, October 9 - 11, 2007 Conference on International Agricultural Research for Development, 1-6.
Oyelese, A. O., Taiwo, V. O., Ogunsanmi, A. O. and Faturoti, E. O. (1999). Toxicological effects of cassava peels on haematology, serum biochemistry and tissue pathology of Clarias gariepinus fingerlings. Tropical Veterinarian 17:17-30.
Rainza-Paiva, M. J. T., Ishikawa, C.M., Dostiras, A.A. and Felizando, N.N. (2000). Haematological analysis of “Chara”, Pseudoplatysoma fasciatum in captivity. Responsible aquaculture in the new millennium: Nice, France. Europe Aquaculture Society Special Publication, 28:590-592.
Satheeshkumar, P., Ananthan, G., Kumar, S. D. and Jagadeesan, L. (2011). Haematology and biochemical parameters of different feeding behaviour of teleost fishes from Vellar estuary, India. Comparative Clinical Pathology, 5, 1 – 5.
Semenkovich, C. F. (2011). Disorders of lipid metabolism. 24th edition. Philadelphia. Chap 213Pp
Stockopf, M. K. (1993). Fish medicine. W.B. Saunders Company, Philadelphia, PA. 331 – 348.
Uyanik, F., Eren, M., Afasever, Tuncoku, G. and Kolsuz, A.H. (2001). Changes in some biochemical parameters and organs of broilers exposed to cadmium and the effect of zinc on cadmium-induced alterations. Israel Journal of Veterinary Medicine, 56: 128-134.
Waldroup, P.W. (1997). Calcium and Phosphorus sources for poultry feeds. University of Arkansas, Fayetteville, AR USA, 21Pp.